Serial dilution is the stepwise dilution of a substance in solution. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. A serial dilution is the stepwise dilution of a substance in solution. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion.
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Thank you! Cancel Post Comment. Cancel Submit Report. Cancel Delete. A serial dilution is a kind of solution dilution. A more exact serial dilution definition is that it is a stepwise dilution of a solution, that is repeated a certain number of times and in which the concentration decreases with each step.
The dilution factor calculator at each step does not have to be constant, but it is for this calculator. In biology and medicine [ edit ] In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample.
Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, , p. Methods in microbiology The cell count or density gradually falls as the serial number increases in each step of serial dilution.
Calculating the overall dilution over the entire series makes it easier to calculate the cell numbers in the primary solution. Objectives of Serial dilution. The serial dilution method is intended to estimate the concentration number of organisms, bacteria, viruses, or colonies of an unknown sample by counting the number of colonies cultured from serial dilutions of the sample. We can readily count the amount of cells present in the sample since the incubated plates from the serial dilution yield an easily countable number of colonies.
The selected sample is diluted through a series of standard quantities of sterile diluents, such as distilled water or 0. Based on the concentration cells contained within the unknown sample, the dilution factor or the number of repeated dilutions is raised.
If a sample is taken from a heavily polluted source, for example, the dilution factor will be raised. If the sample source is less polluted, on the other hand, a low dilution factor should suffice. In laboratories two-fold and ten-fold dilution is used to titer antibodies or prepares diluted analytes.
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